puc19 multi cloning site  (ATCC)

Journal: Virus Research

Article Title: Development of recombinant rabies viruses vectors with Gaussia luciferase reporter based on Chinese vaccine strain CTN181

doi: 10.1016/j.virusres.2011.05.018

Figure Lengend Snippet: The single strand nucleotide acid and primer sequences used in this study for cloning and RT-PCR.

Article Snippet: First, rCTN-Gluc was constructed as follows: the multi-cloning sites (MCS) of pUC19 vector (NEB, U.S.A.) was replaced to EcoRI–ApaI–BssHII– AACA –EcoRV–PvuI–BglII– polyA –NheI–HindIII, formed by annealing of two artificially synthesized, complemented single DNA chains named MCS-F and MCS-R ( ) to designate pUC19-R. Then, a 1930 nt-length EcoRI–BssHII DNA fragment including the latter half of the M gene, the M–G intergenic region (IGR), the complete G gene was cloned into the EcoRI–BssHII site of pUC19-R to designate subclone pUC19-R′.

Techniques: Clone Assay, Sequencing